Investigation of the two types of cellular connections of Schlemms canal inner wall cells and their role in giant vacuole and pore formation by serial block-face scanning electron microscopyReportar como inadecuado


Investigation of the two types of cellular connections of Schlemms canal inner wall cells and their role in giant vacuole and pore formation by serial block-face scanning electron microscopy


Investigation of the two types of cellular connections of Schlemms canal inner wall cells and their role in giant vacuole and pore formation by serial block-face scanning electron microscopy - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Abstract

PURPOSE: To determine, under flow conditions, whether reduced connections between Schlemm’s canal SC inner wall IW and juxtacanalicular tissue JCT cells play a role in giant vacuole GV formation; and whether decreased amount of cell margin overlap between adjacent IW cells promotes paracellular pore formation using serial block-face scanning electron microscopy SBF-SEM.METHODS: Normal human eyes were immersion-fixed 0 mmHg, N=2 or perfusion-fixed 15 mmHg, N=1. Frontal and radial sections of SC were processed for SBF-SEM. IW and JCT cells, GVs, and pores were 3D-reconstructed. In each IW cell, total number of connections with underlying JCT cells-matrix was determined. Total cell margin length TCML and zero-overlap length ZL of each IW cell were measured to calculate percent zero-overlap length PZL=ZL-TCML. All data were compared between the eyes fixed at 0 and 15 mmHg.RESULTS: Total number of IW-JCT connections in individual IWs significantly decreased in the eye fixed at 15 mmHg 33±5, N=5 cells compared to those fixed at 0 mmHg 189±12, N=4 cells, p<0.001. The summed GV volume in individual cells significantly increased in the eye fixed at 15mmHg 218.03±19.65 μm3 compared to those fixed at 0 mmHg 82.33±27.22 μm3, p=0.0043. PZL increased 26.68% p=0.001 in the eye fixed at 15mmHg vs. those fixed at 0mmHg, and all paracellular pores were found only in regions where the overlap length was 0 μm.CONCLUSIONS: Cellular connections between IW-JCT and IW-IW cells play a role in GV and pore formation in normal human eyes under flow conditions. Our results provide a baseline for future comparison with primary open angle glaucoma eyes.

Boston University Theses and Dissertations -



Autor: Lai, Julia - -

Fuente: https://open.bu.edu/



DESCARGAR PDF




Documentos relacionados