Role of Transient Receptor Potential Ion Channels and Evoked Levels of Neuropeptides in a Formaldehyde-Induced Model of Asthma in Balb-c MiceReportar como inadecuado




Role of Transient Receptor Potential Ion Channels and Evoked Levels of Neuropeptides in a Formaldehyde-Induced Model of Asthma in Balb-c Mice - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Objective

Asthma is a complex pulmonary inflammatory disease characterized by the hyper-responsiveness, remodeling and inflammation of airways. Formaldehyde is a common indoor air pollutant that can cause asthma in people experiencing long-term exposure. The irritant effect and adjuvant effect are the two possible pathways of formaldehyde promoted asthma.

Methodology-Principal Findings

To explore the neural mechanisms and adjuvant effect of formaldehyde, 48 Balb-c mice in six experimental groups were exposed to a vehicle control; b ovalbumin; c formaldehyde 3.0 mg-m3; d ovalbumin+formaldehyde 3.0 mg-m3; e ovalbumin+formaldehyde 3.0 mg-m3+HC-030031 transient receptor potential ankyrin 1 antagonist; f ovalbumin+formaldehyde 3.0 mg-m3+ capsazepine transient receptor potential vanilloid 1 antagonist. Experiments were conducted after 4 weeks of combined exposure and 1-week challenge with aerosolized ovalbumin. Airway hyper-responsiveness, pulmonary tissue damage, eosinophil infiltration, and increased levels of interleukin-4, interleukin-6, interleukin-1β, immunoglobulin E, substance P and calcitonin gene-related peptide in lung tissues were found in the ovalbumin+formaldehyde 3.0 mg-m3 group compared with the values seen in ovalbumin -only immunized mice. Except for interleukin-1β levels, other changes in the levels of biomarker could be inhibited by HC-030031 and capsazepine.

Conclusions-Significance

Formaldehyde might be a key risk factor for the rise in asthma cases. Transient receptor potential ion channels and neuropeptides have important roles in formaldehyde promoted-asthma.



Autor: Yang Wu, Huihui You, Ping Ma, Li Li, Ye Yuan, Jinquan Li, Xin Ye, Xudong Liu, Hanchao Yao, Ruchong Chen, Kefang Lai , Xu Yang

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados