Evaluation of Albuminated Curcumin as Soluble Drug Form to Control Growth of Cancer Cells in VitroReportar como inadecuado




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Curcumin Curc is wellknown for its anticancer activity, but its poor solubility in aqueous medium isa major concern for little therapeutic outcome.
Therefore, the effort toimprove its bioavailability is a major research interest.
The current studyaimed at conjugation of Curc to serum albumin Alb to increase aqueoussolubility of the former without affecting its drug action on cancer cell linesand primary cells in culture.
Conditions for preparation of albumin-curcuminAlb-Curc conjugate were standardized to obtain pure and stable drug.
Theproduct was obtained in sufficient quantity to test its effect on cells inculture at different doses.
Briefly, the conjugate was prepared by mixing Curcdissolved in DMSO with the Alb dissolved in phosphate buffered saline;conjugate was purified by gel filtration chromatography and was analyzed usingUV-Vis spectroscopy for characteristic peaks of both molecules.
The conjugatewas added to culture medium to identify the effect of conjugate on cell cyclingand apoptosis.
Albuminated curcumin that showed 100-fold higher solubility thanfree Curc was stable and inhibitory to proliferation, induced cell cycle arrestand apoptosis.
The conjugate showed apoptotic effects on endothelial cellsindicating its anti angiogenic property.
Primary fibroblast growth was alsoinhibited but at the higher dose.
The invitro results suggest thatAlb-Curc which is free of insoluble native drug may find application in cancertherapy after appropriate invivo evaluations.


KEYWORDS

Curcumin, Anticancer Drug, Aqueous Solubility, Bioavailability, Albumin Drug Conjugation

Cite this paper

Thomas, C.
, Pillai, L.
and Krishnan, L.
2014 Evaluation of Albuminated Curcumin as Soluble Drug Form to Control Growth of Cancer Cells in Vitro.
Journal of Cancer Therapy, 5, 723-734.
doi: 10.4236-jct.2014.57081.






Autor: Christina Thomas, Lakshmi Sreedharan Pillai, Lissy Krishnan

Fuente: http://www.scirp.org/





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