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Fungicideswhich are not easily degradable have the greatest adverse effects on soilmicrobes. These pesticides negatively affect the growth and multiplication offungi and bacteria and consequently cause the disturbance of the natural soil microbialbalance. In this study two fungicide tolerant isolates of rhizobia; cloverisolate TA1 and peanut isolate 8 were assessed in their capacity to degradeVitavax and Rizolex. The performance of these isolates in fungicidesdegradation was tested using the colorimetric assay for Rizolex and the HPLCanalysis for Vitavax to detect the degradation products. Using HPLC analyses,the control sample showed specific peak indicating the Vitavax presence in themedium. The specific peak did not change in the control samples throughout theexperiment.With the strainTA1 the specific peak of the Vitavax fungicidesstarted to reduce as the incubation time goes on. The Vitavax fungicide did notdegrade completely after 240 hours of incubation with rhizobial isolate. TheRizolex used in this study contained blend of Thiram active ingredient ofRizolex and Tolcofs methyl fungicides in 1:1 ratio. The biodegradation ofRizolex in the liquid media showed the formation of two new intermediates whichwere released into the medium indicating the degradation of the testedfungicide by peanut rhizobial isolate No. 8 in 48 hrs of incubation 45% of thiscompound was degraded. This work shows that the selection of fungicidestolerant rhizobial strains is important to protect the rhizobial inoculantsfrom the toxic effect of the pesticides.



KEYWORDS

Fungicides, Rhizobia, Biodegradation, HPLC Analyses

Cite this paper

Moawad, H. , El-Rahim, W. , Shawky, H. , Higazy, A. and Daw, Z. 2014 Evidence of Fungicides Degradation by Rhizobia. Agricultural Sciences, 5, 618-624. doi: 10.4236-as.2014.57065.





Autor: Hassan Moawad, Wafaa M. Abd El-Rahim, Haitham Shawky, Aziz M. Higazy, Zakaria Y. Daw

Fuente: http://www.scirp.org/



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