Microsporidia and Its Relation to Crohns Disease. A Retrospective StudyReportar como inadecuado




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Background

The cause of Crohn-s Disease CD remains unknown.
Recently a decrease in the global lymphocyte population in the peripheral blood of CD patients has been reported.
This decrease was more evident in γδ T lymphocytes, especially γδ CD8+T subsets.
Furthermore, a decrease of IL-7 was also observed in these patients.
We propose the hypothesis that microsporidia, an obligate intracellular opportunistic parasite recently related to fungi, in CD patients can take advantage of the lymphocytes and IL-7 deficits to proliferate and to contribute to the pathophysiology of this disease.

Methods and Findings

In this case-control study, serum samples were collected from 36 CD patients and from 36 healthy individuals controls, IgE and IgG anti-Encephalitozoon antibodies were determined by ELISA; and forty-four intestinal tissue samples were analyzed through real time Polymerase Chain Reaction PCR, twenty CD patients, nine with others diseases and 15 healthy subjects.We observed that IgE anti-Encephalitozoon levels were significantly higher in patients with CD: 0.386±0.256 vs control group, 0.201±0.147, P<0.001.
However, IgG anti-Encephalitozoon values were significantly lower in CD patients: 0.361±0.256 vs control group, 0.876±0.380, P<0.001.
In the group of CD patients, 6-20 30% were positive by real time PCR for microsporidia and, all the patients of the control group were negative by real time PCR.

Conclusions

These results suggest that CD patients are a group at risk for microsporidiasis and, moreover that microsporidia may be involved as a possible etiologic factor of CD.



Autor: Juan C.
Andreu-Ballester , Carlos Garcia-Ballesteros, Victoria Amigo, Ferran Ballester, Rafael Gil-Borrás, Ignacio Catalán-Serr


Fuente: http://plos.srce.hr/





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