The Genome Sequence of Lone Star Virus, a Highly Divergent Bunyavirus Found in the Amblyomma americanum TickReportar como inadecuado




The Genome Sequence of Lone Star Virus, a Highly Divergent Bunyavirus Found in the Amblyomma americanum Tick - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Viruses in the family Bunyaviridae infect a wide range of plant, insect, and animal hosts.
Tick-borne bunyaviruses in the Phlebovirus genus, including Severe Fever with Thrombocytopenia Syndrome virus SFTSV in China, Heartland virus HRTV in the United States, and Bhanja virus in Eurasia and Africa have been associated with acute febrile illness in humans.
Here we sought to characterize the growth characteristics and genome of Lone Star virus LSV, an unclassified bunyavirus originally isolated from the lone star tick Amblyomma americanum.
LSV was able to infect both human HeLa and monkey Vero cells.
Cytopathic effects were seen within 72 h in both cell lines; vacuolization was observed in infected Vero, but not HeLa, cells.
Viral culture supernatants were examined by unbiased deep sequencing and analysis using an in-house developed rapid computational pipeline for viral discovery, which definitively identified LSV as a phlebovirus.
De novo assembly of the full genome revealed that LSV is highly divergent, sharing <61% overall amino acid identity with any other bunyavirus.
Despite this sequence diversity, LSV was found by phylogenetic analysis to be part of a well-supported clade that includes members of the Bhanja group viruses, which are most closely related to SFSTV-HRTV.
The genome sequencing of LSV is a critical first step in developing diagnostic tools to determine the risk of arbovirus transmission by A.
americanum, a tick of growing importance given its expanding geographic range and competence as a disease vector.
This study also underscores the power of deep sequencing analysis in rapidly identifying and sequencing the genomes of viruses of potential clinical and public health significance.



Autor: Andrea Swei, Brandy J.
Russell, Samia N.
Naccache, Beniwende Kabre, Narayanan Veeraraghavan, Mark A.
Pilgard, Barbara J.
B.
Johns


Fuente: http://plos.srce.hr/





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