Dermal Mesenchymal Stem Cells DMSCs Inhibit Skin-Homing CD8 T Cell Activity, a Determining Factor of Vitiligo Patients’ Autologous Melanocytes Transplantation EfficiencyReportar como inadecuado




Dermal Mesenchymal Stem Cells DMSCs Inhibit Skin-Homing CD8 T Cell Activity, a Determining Factor of Vitiligo Patients’ Autologous Melanocytes Transplantation Efficiency - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

We here investigated the efficiency of autologous melanocyte transplantation of 23 vitiligo patients by focusing on perilesional skin homing CD8+ T lymphocytes, and studied the potential effect of dermal mesenchymal stem cells DMSCs on CD8+ T cell activities in vitro. Out of 23 patients with the autologous melanocyte transplantation, 12 patients 52.17% had an excellent re-pigmentation, 6 patients 26.09% had a good re-pigmentation, 5 patients 21.74% had a fair or poor re-pigmentation. CD8+ T cells infiltrating was observed in the perilesional vitiligo area of all patients. Importantly, the efficiency of the transplantation was closely associated with skin-homing CD8+ T cell activities. The patients with high number of perilesional CD8+ T cells or high level of cytokines-chemokines were associated with poor re-pigmentation efficiency. For in-vitro experiments, we successfully isolated and characterized human DMSCs and skin-homing CD8+ T cells. We established DMSCs and CD8+ T cell co-culture system, where DMSCs possessed significant inhibitory effects against skin homing CD8+ T lymphocytes. DMSCs inhibited CD8+ T cells proliferation, induced them apoptosis and regulated their cytokines-chemokines production. Our results suggest that vitiligo patients’ autologous melanocytes transplantation efficiency might be predicted by perilesional skin-homing CD8+ T cell activities, and DMSCs might be used as auxiliary agent to improve transplantation efficacy.



Autor: Miao-ni Zhou , Zhi-qing Zhang , Ji-long Wu, Fu-quan Lin, Li-fang Fu, Sui-quan Wang, Cui-ping Guan, Hong-lin Wang, Aie Xu

Fuente: http://plos.srce.hr/



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