A Mouse Model for Studying the Clearance of Hepatitis B Virus In Vivo Using a Luciferase ReporterReportar como inadecuado




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Hepatitis B virusHBV infection remains a global problem, despite the effectiveness of the Hepatitis B vaccine in preventing infection. The resolution of Hepatitis B virus infection has been believed to be attributable to virus-specific immunity. In vivo direct evaluation of anti-HBV immunity in the liver is currently not possible. We have developed a new assay system that detects HBV clearance in the liver after the hydrodynamic transfer of a reporter gene and over-length, linear HBV DNA into hepatocytes, followed by bioluminescence imaging of the reporter gene Fluc. We employed bioluminescence detection of luciferase expression in HBV-infected hepatocytes to measure the Hepatitis B core antigen HBcAg-specific immune responses directed against these infected hepatocytes. Only HBcAg-immunized, but not mock-treated, animals decreased the amounts of luciferase expression, HBsAg and viral DNA from the liver at day 28 after hydrodynamic infection with over-length HBV DNA, indicating that control of luciferase expression correlates with viral clearance from infected hepatocytes.



Autor: Sheng-qiang Liang , Juan Du , Hu Yan, Qian-qian Zhou, Yong Zhou, Zhen-nan Yuan, Shao-duo Yan, Qiu-xia Fu, Xiao-hui Wang, Shuai-zh

Fuente: http://plos.srce.hr/



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