Promotive Effects of Yokukansan, a Traditional Japanese Medicine, on Proliferation and Differentiation of Cultured Mouse Cortical OligodendrocytesReportar como inadecuado




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Effects of yokukansan, a traditional Japanese medicine, on proliferationand differentiation of oligodendrocytes were examined using purified mousecortical oligodendrocyte precursor cells OPCs. OPCs were cultured for fourdays, and proliferation was evaluated by counting A2B5 a specific antibody toOPC-reactive cells on the second day of cell culture. Differentiation from OPCto oligodendrocyte was evaluated by counting O4 a specific antibody to detectdifferentiated cells in various stages-reactive cells on the fourth day ofculture. The effects of yokukansan final concentration: 100 or 200 μg-ml onproliferation and differentiation were examined by adding it to the medium forfour days. Yokukansan increased not only the number of A2B5-positive cells onthe second day but also the number of O4-positive cells on the fourth daycompared to those in the corresponding controls. A WST-8 assay was used toidentify active components from seven components of Uncaria Hook UH, one ofthe constituent galenicals of yokukansan. Geissoschizine methyl ether GM: 0.1- 3.0 μM was identified by this screening assay and increased the number ofA2B5-positive cells on the second day and O4-positive cells on the fourth dayas yokukansan did. These results suggest that yokukansan promotes theproliferation and differentiation of oligodendrocytes, and also that GMcontained in UH is one of active components responsible for this effect ofyokukansan.

KEYWORDS

Yokukansan, Oligodendrocyte, Proliferation, Differentiation, Geissoschizine Methyl Ether

Cite this paper

Ueki, T. , Ikarashi, Y. , Kawakami, Z. , Mizoguchi, K. and Kase, Y. 2014 Promotive Effects of Yokukansan, a Traditional Japanese Medicine, on Proliferation and Differentiation of Cultured Mouse Cortical Oligodendrocytes. Pharmacology & Pharmacy, 5, 670-680. doi: 10.4236-pp.2014.57077.





Autor: Toshiyuki Ueki, Yasushi Ikarashi, Zenji Kawakami, Kazushige Mizoguchi, Yoshio Kase

Fuente: http://www.scirp.org/



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