Suppression of Core 1 Gal-Transferase Is Associated with Reduction of TF and Reciprocal Increase of Tn, sialyl-Tn and Core 3 Glycans in Human Colon Cancer CellsReportar como inadecuado




Suppression of Core 1 Gal-Transferase Is Associated with Reduction of TF and Reciprocal Increase of Tn, sialyl-Tn and Core 3 Glycans in Human Colon Cancer Cells - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

It has long been presumed, though with surprisingly little evidence, a competition between Core 1 Gal-transferase C1GalT, Core 3 GlcNAc-transferase C3GnT and sialyl-transferase ST6GalNAc-T for elongation of O-linked mucin-type glycans initiated with GalNAcα-Ser-Thr. This study tested this presumption by selective suppression of one of these glycosyltransferases and then analysed the expressions of the enzymatic products of the other three glycosyltransferases. It was found that siRNA suppression of C1GalT markedly reduced the expression of Galβ1,3GalNAcα- Core 1 and in the meantime increased the expressions of sialyl-GalNAcα- sialyl-Tn, GalNAcα- Tn and GlcNAcβ1,3GalNAcα- Core 3-associated glycans in human colon cancer HT29 and SW620 cells. This supports a competitive modification of the GalNAcα-Ser-Thr between C1GalT, C3GnT and ST6GalNAc-T in O-glycan biosynthesis. As Tn, TF and sialyl-Tn are oncofetal antigens and are over-expressed in most human cancers, this information is useful for the development of glycosyltransferase-targeted therapeutic strategies for cancer treatment.



Autor: Hannah Barrow , Benjamin Tam , Carrie A. Duckworth , Jonathan M. Rhodes, Lu-Gang Yu

Fuente: http://plos.srce.hr/



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