Blastomyces dermatitidis Antibody Responses in Serial Serum Specimens from Dogs with Blastomycosis: Comparison of Different Yeast Lysate AntigensReportar como inadecuado




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The systemic fungal organism, Blastomyces dermatitidis causes blastomycosis in animals and hu-mans. This study was designed to evaluate antibody detection in 55 serial serum specimens from 9 dogs with blastomycosis using B. dermatitidis yeast lysate antigens produced from two human isolates B5896; B5931 and two dog isolates ERC-2; T-58 with the indirect enzyme linked im-munosorbent assay ELISA; peroxidase system to determine an optimal lysate antigens for use in the ELISA to detect antibody in the dog serum specimens. The mean absorbance values when the lysate antigens were compared with respect to their ability to detect antibody in the day 0 sera from the 9 dogs were 1.024 ERC-2, 1.351 B5896, 1.700 B5931 and 2.084 T-58 respectively. All of the reagents exhibited a high level of sensitivity and in all instances the amount of antibody declined as the time interval post-treatment increased, but the T-58 lysate prepared from the dog isolate from Tennessee was the optimal reagent. We continue to evaluate antigens for B. derma-titidis antibody detection in different immunodiagnostic assays.

KEYWORDS

Blastomycosis, Lysate Antigens, Antibody Detection, ELISA, Serial Dog Serum Specimens

Cite this paper

Shepherd, M. , Lutes, M. and Scalarone, G. 2014 Blastomyces dermatitidis Antibody Responses in Serial Serum Specimens from Dogs with Blastomycosis: Comparison of Different Yeast Lysate Antigens. Journal of Biosciences and Medicines, 2, 67-73. doi: 10.4236-jbm.2014.22011.





Autor: Mara Shepherd, Misty Lutes, Gene Scalarone

Fuente: http://www.scirp.org/



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