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In this study, we analyzed viral metagenomes viromes in the sedimentary habitats of three geographically and geologically distinct hadopelagic environments in the northwest Pacific; the Izu-Ogasawara Trench water depth = 9,760 m OG, the Challenger Deep in the Mariana Trench 10,325 m MA, and the forearc basin off the Shimokita Peninsula 1,181 m SH. Virus abundance ranged from 106 to 1011 viruses-cm3 of sediments down to 30 cm below the seafloor cmbsf. We recovered viral DNA assemblages viromes from the hadopelagic sediment samples and obtained a total of 37,458, 39,882, and 70,882 sequence reads by 454 GS FLX Titanium pyrosequencing from the virome libraries of the OG, MA, and SH hadopelagic sediments, respectively. Only 24−30% of the sequence reads from each virome library exhibited significant similarities to the sequences deposited in the public nr protein database E-value <10−3 in BLAST. Among the sequences identified as potential viral genes based on the BLAST search, 95−99% of the sequence reads in each library were related to genes from single-stranded DNA ssDNA viral families, including Microviridae, Circoviridae, and Geminiviridae. A relatively high abundance of sequences related to the genetic markers major capsid protein VP1 and replication protein Rep of two ssDNA viral groups were also detected in these libraries, thereby revealing a high genotypic diversity of their viruses 833 genotypes for VP1 and 2,551 genotypes for Rep. A majority of the viral genes predicted from each library were classified into three ssDNA viral protein categories: Rep, VP1, and minor capsid protein. The deep-sea sedimentary viromes were distinct from the viromes obtained from the oceanic and fresh waters and marine eukaryotes, and thus, deep-sea sediments harbor novel viromes, including previously unidentified ssDNA viruses.



Autor: Mitsuhiro Yoshida , Yoshihiro Takaki, Masamitsu Eitoku, Takuro Nunoura, Ken Takai

Fuente: http://plos.srce.hr/



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