Electroacupuncture Acutely Improves Cerebral Blood Flow and Attenuates Moderate Ischemic Injury via an Endothelial Mechanism in MiceReportar como inadecuado




Electroacupuncture Acutely Improves Cerebral Blood Flow and Attenuates Moderate Ischemic Injury via an Endothelial Mechanism in Mice - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Electroacupuncture EA is a novel therapy based on traditional acupuncture combined with modern eletrotherapy that is currently being investigated as a treatment for acute ischemic stroke. Here, we studied whether acute EA stimulation improves tissue and functional outcome following experimentally induced cerebral ischemia in mice. We hypothesized that endothelial nitric oxide synthase eNOS-mediated perfusion augmentation was related to the beneficial effects of EA by interventions in acute ischemic injury. EA stimulation at Baihui GV20 and Dazhui GV14 increased cerebral perfusion in the cerebral cortex, which was suppressed in eNOS KO, but there was no mean arterial blood pressure MABP response. The increased perfusion elicited by EA were completely abolished by a muscarinic acetylcholine receptor mAChR blocker atropine, but not a β-adrenergic receptor blocker propranolol, an α-adrenergic receptor blocker phentolamine, or a nicotinic acetylcholine receptor nAChR blocker mecamylamine. In addition, EA increased acetylcholine ACh release and mAChR M3 expression in the cerebral cortex. Acute EA stimulation after occlusion significantly reduced infarct volume by 34.5% when compared to a control group of mice at 24 h after 60 min-middle cerebral artery occlusion MCAO moderate ischemic injury, but not 90-min MCAO severe ischemic injury. Furthermore, the impact of EA on moderate ischemic injury was totally abolished in eNOS KO. Consistent with a smaller infarct size, acute EA stimulation led to prominent improvement of neurological function and vestibule-motor function. Our results suggest that acute EA stimulation after moderate focal cerebral ischemia, but not severe ischemia improves tissue and functional recovery and ACh-eNOS-mediated perfusion augmentation might be related to these beneficial effects of EA by interventions in acute ischemic injury.



Autor: Ji Hyun Kim, Kyung Ha Choi, Young Jung Jang, Sun Sik Bae, Byung-Cheul Shin, Byung Tae Choi , Hwa Kyoung Shin

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados