High Prevalence of Ciprofloxacin Resistance in Community Associated Staphylococcus aureus in a Tertiary Care Indian HospitalReportar como inadecuado




High Prevalence of Ciprofloxacin Resistance in Community Associated Staphylococcus aureus in a Tertiary Care Indian Hospital - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

We have studied the nature of ciprofloxacin resistancein methicillin sensitive and resistant Staphylococcus aureus amongpatients in a tertiary care hospital in Bengaluru, South India. All theisolates were highly resistant to ciprofloxacin. Molecular characterization ofthese samples performed using Staphylococcal Cassette Chromosome typing andmultilocus sequence typing showed that 37.5% of total isolates and 59% of MRSAwere sequence type ST772 and the rest were other STs. This indicates highprevalence of CA-MRSA in this tertiary care hospital serving the Indiancommunity. Mutations responsible for ciprofloxacin resistance among these isolatesin DNA gyrase gyrA and gyrB and topoisomerase IV grlA and grlB were analyzed by PCR amplification of specific fragments andsequencing. We found that for ST772 and five other STs present in thiscollection, single mutation in the gyrA gene, Ser-84→Leu, was sufficientfor the high resistance. In vitro generation of ciprofloxacin resistancein two sensitive ST772 isolates by exposure to increasing antibioticconcentrations also resulted in the same single mutation of gyrA. Thefactors responsible for high ciprofloxacin resistance are varied and aredependent on the genetic background of the isolates and the environment. Thisis the first report on the mechanism of ciprofloxacin resistance among the mostprevalent Indian CA-MRSA.



KEYWORDS

Ciprofloxacin Resistance; Indian ST772 Staphylococcus Aureus; gyrA Mutations

Cite this paper

B. Chakrakodi, S. Prabhakara, S. Nagaraj, J. Etienne and G. Arakere -High Prevalence of Ciprofloxacin Resistance in Community Associated Staphylococcus aureus in a Tertiary Care Indian Hospital,- Advances in Microbiology, Vol. 4 No. 2, 2014, pp. 133-141. doi: 10.4236-aim.2014.42018.





Autor: Bhavya Chakrakodi, Sushma Prabhakara, Savitha Nagaraj, Jerome Etienne, Gayathri Arakere

Fuente: http://www.scirp.org/



DESCARGAR PDF




Documentos relacionados