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Liquid and semi-solid culturemedia are used to maintain and proliferate bacteria, fungi, and Oomycetes forresearch in microbiology and plant pathology. In this study, a comparison wasmade between soybean milk medium, also referred to as soymilk, and mediatraditionally used for culturing soybean pathogens to determine if soymilkmedium was an effective medium for growth of Colletotrichum truncatum, Fusarium virguliforme, Macrophomina phaseolina, Passalora sojina, Phomopsis longicolla, Phytophthorasojae, Pythium irregulare, Rhizoctonia solani, and Sclerotinia sclerotiorum. Based onradial mycelial colony growth rates, C.sojina grewsignificantly P < 0.05 faster on soymilk dextrose agarSDA than on V-8 agar, and C. truncatum and F. virguliforme grew significantly P < 0.05faster on SDA than potato dextrose agar PDA. Significantly P < 0.001 greater masses of sclerotia were produced by S. sclerotiorum grown on SDA as comparedto PDA. Soymilk used with agar or used alone as a broth may be anoption for replacing more expensive processed culture media.



KEYWORDS

Soybean Pathogens; Culture Media; Glycine max

Cite this paper

Y. Xiang, T. Herman and G. Hartman -Utilizing Soybean Milk to Culture Soybean Pathogens,- Advances in Microbiology, Vol. 4 No. 2, 2014, pp. 126-132. doi: 10.4236-aim.2014.42017.





Autor: Yiwen Xiang, Theresa Herman, Glen L. Hartman

Fuente: http://www.scirp.org/



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