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Toll-like receptor TLR signaling is thought to beone of the most important pathways initiating periodontitis onset. We havepreviously reported that the TLR signaling pathway is upregulated in periodontitis-affected gingival tissues by microarray pathway frequency analysis. The aimof the present study was to quantitatively analyze specific upregulated genes inthe TLR signaling pathway, as compared to healthy controls. Healthy andperiodontitis-affected gingival tissues weretaken from distinct sites of 3 patients with severe chronicperiodontitis. Total RNAs from 6 gingival tissue samples were used for microarray.Samples were taken from 14 chronic periodontitis patients and 14 healthyindividuals for quantitative reverse transcriptionreal-time polymerase chain reaction qRT-PCR analysis. Data-mininganalyses, such as pathway analyses, were performed and significant biologicalpathways in periodontitis were identified. In addition, qRT-PCR analysis wasperformed for 5 genes—cluster of differentiation 14 CD14, lymphocyte antigen96 MD-2, interleukin-1 beta IL-1β,interleukin 8 IL-8, and chemokine ligand 9 CXCL-9, which are associatedwith TLR signaling, in order to confirm theresults of pathway analysis. qRT-PCR verified that the transcripts for5 genes in the TLR signaling pathway were significantly upregulated MD-2 p = 0.0082, CD14 p = 0.0322, IL-1β p = 0.0126, IL-8 p = 0.0438, CXCL-9 p = 0.0325,which was consistent with pathway analyses. We confirmed upregulated MD-2 gene expression levels and associated TLRpathway gene expression, including CD14, IL-1β, IL-8 and CXCL-9, in periodontitis-affected gingival tissues, ascompared with healthy controls.

KEYWORDS

TLR Signaling Pathway; Gene Expression; Microarray; qRT-PCR

Cite this paper

Abe, D. , Kubota, T. , Morozumi, T. and Yoshie, H. 2014 Upregulated genes in toll-like receptor TLR signaling pathway in periodontitis-affected gingival tissues. Open Journal of Stomatology, 4, 22-28. doi: 10.4236-ojst.2014.41005.





Autor: Daisuke Abe, Takehiko Kubota, Toshiya Morozumi, Hiromasa Yoshie

Fuente: http://www.scirp.org/



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