Antiplatelet Agents Can Promote Two-Peaked Thrombin Generation in Platelet Rich Plasma: Mechanism and Possible ApplicationsReportar como inadecuado

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Thrombin generation assay is a convenient and widely used method for analysis of the blood coagulation system status. Thrombin generation curve TGC is usually bell-shaped with a single peak, but there are exceptions. In particular, TGC in platelet-rich plasma PRP can sometimes have two peaks.


We sought to understand the mechanism underlying the occurrence of two peaks in the PRP thrombin generation curve.


Tissue factor-induced thrombin generation in PRP and platelet-poor plasma PPP was monitored using continuous measurement of the hydrolysis rate of the thrombin-specific fluorogenic substrate Z-Gly-Gly-Arg-AMC. Expression of phosphatidylserine PS and CD62P on the surface of activated platelets was measured by flow cytometry using corresponding fluorescently labeled markers.


The addition of the P2Y12 receptor antagonist MeS-AMP 160 µM, 83 nM prostaglandin E1 PGE1, or 1.6% DMSO to PRP caused the appearance of two peaks in the TGC. The PS exposure after thrombin activation on washed platelets in a suspension supplemented with DMSO, PGE1 or MeS-AMP was delayed, which could indicate mechanism of the second peak formation. Supplementation of PRP with 1.6% DMSO plus 830 nM PGE1 mediated the disappearance of the second peak and decreased the amplitude of the first peak. Increasing the platelet concentration in the PRP promoted the consolidation of the two peaks into one.


Procoagulant tenase and prothrombinase complexes in PRP assemble on phospholipid surfaces containing PS of two types - plasma lipoproteins and the surface of activated platelets. Thrombin generation in the PRP can be two-peaked. The second peak appears in the presence of platelet antagonists as a result of delayed PS expression on platelets, which leads to delayed assembly of the membrane-dependent procoagulant complexes and a second wave of thrombin generation.

Autor: Ivan D. Tarandovskiy , Elena O. Artemenko, Mikhail A. Panteleev, Elena I. Sinauridze, Fazoil I. Ataullakhanov



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