Human Bocavirus Infection in Children with Acute Respiratory Infection in Nairobi, KenyaReportar como inadecuado




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Background: Acute respiratory infection ARI is a leading cause of morbidity and mortality in children under five years of age in developing countries with viruses contributing significantly to this problem. The recently identified parvovirus, Human Bocavirus HBoV, has also been associated with ARI. Objective: To determine the frequency of HBoV in patients with ARI. Materials and Methods: Samples from 125 consenting patients with influenza like illness signs and symptoms were collected. DNA was extracted from these samples using the QIAamp DNA blood mini kit Qiagen, Germany. Conventional PCR was carried out and the amplicons were examined in 2% agarose gels stained with ethidium bromide. This was followed by sequencing of the HBoV positive samples. Results: Twenty one 16.8% patients were found to have HBoV infection. Males n = 61.9% were mainly infected with HBoV. Local HBoV strains had 98.9% - 100% similarities and were found to cluster together with other strains obtained elsewhere. Conclusion: These findings suggest that HBoV plays a role in respiratory tract infections in children in Kenya just like it has been found elsewhere. It also sheds light on multiple infections associated with HBoV infections in Kenya.

KEYWORDS

Acute Respiratory Infection ARI; Human Bocavirus HBoV; Polymerase Chain Reaction PCR

Cite this paper

S. Symekher, G. Gachara, J. Simwa, J. Gichogo, M. Rotich, M. Ng’ayo and J. Magana -Human Bocavirus Infection in Children with Acute Respiratory Infection in Nairobi, Kenya,- Open Journal of Medical Microbiology, Vol. 3 No. 4, 2013, pp. 234-238. doi: 10.4236-ojmm.2013.34035.





Autor: Samwel Morris Lifumo Symekher, George Gachara, James Maylor Simwa, Jane Gichogo, Moses Rotich, Musa Otieno Ng’ayo, Japheth Maga

Fuente: http://www.scirp.org/



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