A mutation in protein kinase C-gamma alters SNC neuron morphology and decreases synaptic vesicles in dopaminergic striatal terminals in the AS-AGU ratReportar como inadecuado




A mutation in protein kinase C-gamma alters SNC neuron morphology and decreases synaptic vesicles in dopaminergic striatal terminals in the AS-AGU rat - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Aspontaneous mutation in the Albino Swiss AS rat has been shown to be a singlepoint mutation agu in the genecoding for the gamma isoform of protein kinase C PKC-γ. The characteristics of the mutant include movement disorders, afailure to release dopamine in the striatum and elevations of molecules such asparkin and ubiquitin in the substantia nigra pars compacta SNC. This presentstudy examined SNC cell bodies and dopaminergic synaptic terminals within thecaudate-putamen. Cell volume and nuclear volume were reduced in the AS-AGUmutant compared to the AS control, but the volume fractions of mitochondria andrough endoplasmic reticulum were significantly higher. No Lewy bodies werepresent in the mutant, although microglia were found adjacent to some SNC cells.Dopaminergic terminals were identified in the caudate-putamen by electron microscopywith low-glutaraldehyde fixation and immunohistochemistry for tyrosinehydroxylase using immuno-gold visualisation. AS-AGU mutant rats had less thanhalf of the synaptic vesicles of AS controls; this was not only true of-readily-releasable- zones adjacent to the synaptic cleft but also -storagepool- zones. The findings support the hypothesis that the initial bar todopamine availability in the striatum is the reduced release, with nigral celldeath being a later phenomenon.

 

KEYWORDS

PKC-Gamma; Nigral DA Neurons and Terminals; PKC-Gamma; SNC Neurons; Dopaminergic Terminals

Cite this paper

Al-Kushi, A. , Russell, D. and Payne, A. 2013 A mutation in protein kinase C-gamma alters SNC neuron morphology and decreases synaptic vesicles in dopaminergic striatal terminals in the AS-AGU rat. Journal of Biomedical Science and Engineering, 6, 1129-1136. doi: 10.4236-jbise.2013.612141.





Autor: Abdullah Glil Al-Kushi, David Russell, Anthony Philip Payne

Fuente: http://www.scirp.org/



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