Wound Closure on the Neonatal Rat Skin II. The Potential Ability of Epidermis to Close Small-Sized Wounds Independently of the Underlying DermisReportar como inadecuado




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Reepithelialization of skin which comprises epidermisand dermis has not been fully elucidated due to the complexity of theparticipants as well as the interactions therein. In this study, the intrinsicroles and behaviors of epidermis itself during wound closureon neonatal rat skin were explored by developing and utilizing a novel in vivo wound model, termed -shallowincisional wound- in which the injury of dermis was minimized. The shallowwounds were closed by 12 h postwounding PW by the migration of thewound-marginal epidermal sheets in which activated myosin light chain p-MLCwas predominantly detected at the lateral plasma membrane of individual cells. Bylocal administration of Rho-associated protein kinase ROCK inhibitor Y27632,p-MLC disappeared at the wound margin and wounds were not closed by 12 h PW. Inhibition of Rac 1 by NSC23766 alsoresulted in hold of wound closure by 12 h PW, though NSC23766 somewhat slowlyacted on p-MLC expression. These results suggest that, without joining ofdermis, epidermal cells have a potential ability of closing wounds by activeepithelial sheet movement integrated by Rho family small GTPases-dependentextension and contraction of the individual cell bodies.

KEYWORDS

Wound Closure; Reepithelialization; Rat; Neonate; Epidermis; Migration; Actomyosin; Rho Family Small GTPases

Cite this paper

M. Arai, T. Matsuzaki and S. Ihara -Wound Closure on the Neonatal Rat Skin II. The Potential Ability of Epidermis to Close Small-Sized Wounds Independently of the Underlying Dermis,- CellBio, Vol. 2 No. 4, 2013, pp. 257-266. doi: 10.4236-cellbio.2013.24028.





Autor: Mary Arai, Takashi Matsuzaki, Setsunosuke Ihara

Fuente: http://www.scirp.org/



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