Wound Closure on the Neonatal Rat Skin I. The Modulation of the Thickness of Epidermis at the Closing Incisional WoundsReportar como inadecuado




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Full-thickness incisional wounds were made on the dorsalskin of 1-day-old rats to elucidate the mechanism of the fluctuationof the epidermal thickness after the wound closure. The thickness of theepidermis covering the wound reached a peak around 96 h post-wounding PW, andbecame thinner thereafter. The analyses of the cell proliferation and apoptosisat the epidermal wound regions revealed that the rate of TUNEL-positive cellsthat displays the cells undergoing apoptosis increased as the epidermis becamethinner around 120 h PW. Next, immunohistochemical analyses using antibodiesagainst keratinocyte differentiation marker proteins indicated that the delayor interruption of the spinous to granular transition from 96 to 120 h PW mightresult in the epidermal thickening in the wound region. Third, the regionundyed with anti-caspase-14 antibody extended downward in the thickenedepidermis by 96 h PW, and in turn, it became intensely and widely stained with thisantibody in the thinning epidermis by 120 h PW. Taken together, it is likelythat the delay and acceleration of the terminal differentiation, includingcornification of the epidermal keratinocytes may coordinately cause the fluctuationof the thickness of the epidermis at the wound site in rat neonates.

KEYWORDS

Wound Healing; Reepithelialization; Rat; Neonate; Epidermis; Terminal Differentiation; Cornification

Cite this paper

M. Arai, T. Matsuzaki and S. Ihara -Wound Closure on the Neonatal Rat Skin I. The Modulation of the Thickness of Epidermis at the Closing Incisional Wounds,- CellBio, Vol. 2 No. 4, 2013, pp. 248-256. doi: 10.4236-cellbio.2013.24027.





Autor: Mary Arai, Takashi Matsuzaki, Setsunosuke Ihara

Fuente: http://www.scirp.org/



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