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Drug target factor complex identification is necessary forevidence based drug discovery. Previous study showed that using small chemicalimmobilized magneticnanobeads the chemical target factors were effectively purified and identified. Here we succeeded to purify thechemical target factor complex, so called cofactors. Arginine exhibits a variety of biologicalactivities through a complex and highly regulated set of pathways that remainincompletely understood at both the whole-body and the cellular levels. The aimof this study is to develop and validate effective purification system for arginine targetcomplex. New argininetarget protein arginine interacting factor 4, AIF4 was purified andidentified. Using recombinant AIF4 protein and arginine-immobilized magneticnanobeads, AIF4 cofactor, AIF4-BP1, were purified. Interaction of AIF4 andAIF4-BP1 was detected in arginine-dependent manner, suggesting arginine receptor complex formation. This nanobeadstechnology is more than30-fold efficient purification efficient than general purification technology.


Arginine; Nanobeads

Cite this paper

Umeda, M. , Uebi, T. , Maekawa, N. , Masaike, Y. , Handa, H. and Imai, T. 2013 Effective cofactor complex purification using nanobeads. Journal of Biosciences and Medicines, 1, 5-10. doi: 10.4236-jbm.2013.13002.

Autor: Makoto Umeda, Tatsuya Uebi, Naoya Maekawa, Yuka Masaike, Hiroshi Handa, Takeshi Imai

Fuente: http://www.scirp.org/


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