PolyDimethylsiloxane PDMS Affects Gene Expression in PC12 Cells Differentiating into Neuronal-Like CellsReportar como inadecuado




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Introduction

Microfluidics systems usually consist of materials like PMMA - polymethyl methacrylate and PDMS - polydimethylsiloxane and not polystyrene PS, which is usually used for cell culture. Cellular and molecular responses in cells grown on PS are well characterized due to decades of accumulated research. In contrast, the experience base is limited for materials used in microfludics chip fabrication.

Methods

The effect of different materials PS, PMMA and perforated PMMA with a piece of PDMS underneath on the growth and differentiation of PC12 adrenal phaeochromocytoma cells into neuronal-like cells was investigated using cell viability, cell cycle distribution, morphology, and gene expression analysis.

Results-Conclusions

After differentiation, the morphology, viability and cell cycle distribution of PC12 cells grown on PS, PMMA with and without PDMS underneath was the same. By contrast, 41 genes showed different expression for PC12 cells differentiating on PMMA as compared to on PS. In contrast, 677 genes showed different expression on PMMA with PDMS underneath as compared with PC12 cells on PS. The differentially expressed genes are involved in neuronal cell development and function. However, there were also many markers for neuronal cell development and functions that were expressed similarly in cells differentiating on PS, PMMA and PMMA with PDMS underneath. In conclusion, it was shown that PMMA has a minor impact and PDMS a major impact on gene expression in PC12 cells.



Autor: Joanna M. Łopacińska, Jenny Emnéus, Martin Dufva

Fuente: http://plos.srce.hr/



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