Bone Regeneration Enhanced by Antigen-Extracted Xenogeneic Cancellous Bone Graft with rhBMP-2 in Rabbits Mandibular Defect RepairReportar como inadecuado




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The effects of large piece xenogeneic bone which wasseparated from healthy pigs as a scaffold on repair of mandibular defect wasinvestigated and the applicability of antigen-extracted xenogeneic cancellousbone AXCB soaked with rhBMP-2 in bone defect repair was assessed. Mandibulardefects were created in 48 New Zealand Rabbits, and then randomly divided into4 groups, which was grafted in the mandibular defects with AXCB, AXCB soakedwith rhBMP-2, autograft bone, or blank. Equal number of animals from each groupwas classified into three time points 4, 8, and 12 weeks after operation forgross pathological observation, hematoxylin and eosin H & E staining,radiographic examination, and bone density measurement. H & E stainingrevealed that the area percentage of bone regeneration in the group ofAXCB-rhBMP-2 graft was 27.72 ± 4.68, 53.90 ± 21.92,and 77.35 ± 9.83 when at 4, 8, and 12 weeks, which was better than that of autobone graft, prompting that the group of AXCB-rhBMP-2 graft had commendableosteogenic effect. And comparing with the AXCB without rhBMP-2, of which thearea percentage of bone regeneration was only 14.03 ± 5.02,28.49 ± 11.35, and 53.90 ± 21.92, the osteogenic effect of AXCB-rhBMP-2graft was demonstrated to be much better. In the group of AXCB-rhBMP-2 graft, thearea percentage of bone regeneration increased, and the implanted materialswere gradually degraded and replaced by autogenous bone regeneration over time.We concluded that antigen-extracted xenogeneic cancellous bone AXCBgraft soaked with rhBMP-2 had shown excellent osteogenic effect in repair ofbone defects, with good biocompability.

KEYWORDS

Recombinant Human Bone Morphogenetic Protein-2rhBMP-2; Antigen-Extracted Xenogeneic Cancellous Bone AXCB; Defect Repair; Bone Regeneration; Mandible Defect

Cite this paper

Lai, R. , Li, Z. , Zhang, Y. and Zhou, Z. 2013 Bone Regeneration Enhanced by Antigen-Extracted Xenogeneic Cancellous Bone Graft with rhBMP-2 in Rabbits Mandibular Defect Repair. Engineering, 5, 108-113. doi: 10.4236-eng.2013.510B022.





Autor: Renfa Lai, Zejian Li, Ye Zhang, Zhiying Zhou

Fuente: http://www.scirp.org/



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