Resistance after Chronic Application of the HDAC-Inhibitor Valproic Acid Is Associated with Elevated Akt Activation in Renal Cell Carcinoma In VivoReportar como inadecuado




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Targeted drugs have significantly improved the therapeutic options for advanced renal cell carcinoma RCC. However, resistance often develops, negating the benefit of these agents. In the present study, the molecular mechanisms of acquired resistance towards the histone deacetylase HDAC inhibitor valproic acid VPA in a RCC in vivo model were investigated. NMRI:nu-nu mice were transplanted with Caki-1 RCC cells and then treated with VPA 200 mg-kg-day. Controls remained untreated. Based on tumor growth dynamics, the mice were divided into -responders- and -non-responders- to VPA. Histone H3 and H4 acetylation and expression of cell signaling and cell cycle regulating proteins in the RCC mouse tumors were evaluated by Western blotting. Tumor growth of VPA responders was significantly diminished, whereas that of VPA non-responders even exceeded control values. Cdk1, 2 and 4 proteins were strongly enhanced in the non-responders. Importantly, Akt expression and activity were massively up-regulated in the tumors of the VPA non-responders. Chronic application 12 weeks of VPA to Caki-1 cells in vitro evoked a distinct elevation of Akt activity and cancer cells no longer responded with cell growth reduction, compared to the short 2 week treatment. We assume that chronic use of an HDAC-inhibitor is associated with re-activation of Akt, which may be involved in resistance development. Consequently, combined blockade of both HDAC and Akt may delay or prevent drug resistance in RCC.



Autor: Eva Juengel, Jasmina Makarević, Igor Tsaur, Georg Bartsch, Karen Nelson, Axel Haferkamp , Roman A. Blaheta

Fuente: http://plos.srce.hr/



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