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Inthis paper, the relatived mechanism between lipofectamine 2000 mediatedtransmembrane gene delivery and endocytic pathway were investigated. Clathrin and caveolae-mediated endocytic pathwaycontributions to transfection efficiency were studied. The inhibitorsof endocytosis were used to treat HEp-2 cells before lipofectamine 2000-pGFP-N2transfection. Transfection efficiency was evaluated with green fluorescence proteinGFP expression assays. Cell viability and cytotoxicity were evaluated withMTT method. The results indicated that inhibitors of clathrin chlorpromazineor wortmannin and caveolin genistein could reduce the cell transfection efficiency observably.Both clathrin and caveolae-mediated endocytic pathways play important roles intransmembrane gene delivery.


Gene Delivery; Cationic Liposomes; Transfection Efficiency; Endocytic Pathway; Inhibitor

Cite this paper

S. Cui, S. Zhang, H. Chen, B. Wang, Y. Zhao and D. Zhi -The Mechanism of Lipofectamine 2000 Mediated Transmembrane Gene Delivery,- Engineering, Vol. 4 No. 10B, 2012, pp. 172-175. doi: 10.4236-eng.2012.410B045.

Autor: Shaohui Cui, Shubiao Zhang, Huiying Chen, Bing Wang, Yinan Zhao, Defu Zhi



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