Analysis of malaria diagnosis discrepancies between RDTs and microscopy by nested PCRReportar como inadecuado




Analysis of malaria diagnosis discrepancies between RDTs and microscopy by nested PCR - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

A total of 415 samples from febrile children exhibiting either concordant n = 108 or discordant n = 307 results between microscopy, the gold standard diagnostic test, and two Rapid Diagnostic Tests RDTs, OptiMAL-IT?pLDH and Acon?HRP2, were analysed using polymerase chain reaction PCR from May to December 2011 in Gabon. The aim of the study was to analyse these discrepancies using poly-merase chain reaction PCR. Nested PCR targeting the Plasmodium ssrRNA gene was used to distinguish P. falciparum, P. malariae and P. ovale. Plasmodium falciparum was the only malaria species identified. Discrepancies frequently involved samples that were negative by microscopy and positive by Acon?HRP2 90% or Optimal-it?86%. The PCR assay detected submicroscopic infection in almost 23% of the microscopy-negative samples, whereas plasmodial DNA was not found in 77% of the Acon?HRP2 positive-microscopy negative samples. Although results obtained with Optimal-it? were more frequently concordant with those of PCR genotyping, the low specificity of Optimal-iT?for non-falciparum malaria parasite detection resulted in a high proportion of false negative RDTs 90% and a high frequency of tests with faint line intensity. The present study highlights the specific attributes of the different methods used to identify malaria parasite below the microscopy level of detection. RDT results that were discordant with either microscopy or PCR as the gold standard could represent a challenge for rapid, accurate fever case management in malaria endemic areas. It is necessary to pursue the development of more precise and more sensitive point-of-care diagnostic tools for malaria.

KEYWORDS

Plasmodium falciparum; RDTs; Microscopy; PCR; Gabon

Cite this paper

Mawili-Mboumba, D. , Bouyou-Akotet, M. , Mbouoronde, C. and Kombila, M. 2013 Analysis of malaria diagnosis discrepancies between RDTs and microscopy by nested PCR. Journal of Biomedical Science and Engineering, 6, 967-972. doi: 10.4236-jbise.2013.610120.





Autor: Denise Patricia Mawili-Mboumba, Marielle Karine Bouyou-Akotet, Christelle Offouga Mbouoronde, Maryvonne Kombila

Fuente: http://www.scirp.org/



DESCARGAR PDF




Documentos relacionados