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In the present study, the morphological and ultrastructural characteristics of mesenchymal stem cells MSCs induced towards osteogenic, adipogenic, and chondrogenic lineages were investigated. The main objective of this paper was to focus on the differentiation capacity of ovine oMSCs and equine MSCs eMSCs. Bone marrow BM MSCs were isolated from ovine and equine patients, expanded in monolayer culture and induced into osteogenic, adipogenic and chondrogenic differentiation. For chondrogenic differentiation, cells were cultured in micromass culture. Also, their ultrastructural phenotypes were studied by transmission electron microscopy TEM. This study was aimed to provide an indepth morphological description of BM-derived ovine and equine MSCs induced towards three lineages, it demonstrated that TEM analysis is useful in elucidating detailed structural information.

KEYWORDS

Ultrastructure; Mesenchymal Stem Cells MSCs; TEM; Differentiation; Equine; Ovine

Cite this paper

Ozen, A. , Gul Sancak, I. , Von Rechenberg, B. and Koch, S. 2013 Ultrastructural Characteristics of Sheep and Horse Mesenchymal Stem Cells MSCs. Microscopy Research, 1, 17-23. doi: 10.4236-mr.2013.13004.





Autor: Asuman Ozen, Irem Gul Sancak, Brigitte Von Rechenberg, Sabine Koch

Fuente: http://www.scirp.org/



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