Intramyocardial Delivery of Mesenchymal Stem Cell-Seeded Hydrogel Preserves Cardiac Function and Attenuates Ventricular Remodeling after Myocardial InfarctionReportar como inadecuado




Intramyocardial Delivery of Mesenchymal Stem Cell-Seeded Hydrogel Preserves Cardiac Function and Attenuates Ventricular Remodeling after Myocardial Infarction - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Background

To improve the efficacy of bone marrow-derived mesenchymal stem cell MSC therapy targeted to infarcted myocardium, we investigated whether a self-setting silanized hydroxypropyl methylcellulose Si-HPMC hydrogel seeded with MSC MSC+hydrogel could preserve cardiac function and attenuate left ventricular LV remodeling during an 8-week follow-up study in a rat model of myocardial infarction MI.

Methodology-Principal Finding

Si-HPMC hydrogel alone, MSC alone or MSC+hydrogel were injected into the myocardium immediately after coronary artery ligation in female Lewis rats. Animals in the MSC+hydrogel group showed an increase in cardiac function up to 28 days after MI and a mid-term prevention of cardiac function alteration at day 56. Histological analyses indicated that the injection of MSC+hydrogel induced a decrease in MI size and an increase in scar thickness and ultimately limited the transmural extent of MI. These findings show that intramyocardial injection of MSC+hydrogel induced short-term recovery of ventricular function and mid-term attenuation of remodeling after MI.

Conclusion-Significance

These beneficial effects may be related to the specific scaffolding properties of the Si-HPMC hydrogel that may provide the ability to support MSC injection and engraftment within myocardium.



Autor: Eva Mathieu, Guillaume Lamirault, Claire Toquet, Pierre Lhommet, Emilie Rederstorff, Sophie Sourice, Kevin Biteau, Philippe Hulin

Fuente: http://plos.srce.hr/



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