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Croatian medical journal, Vol.52 No.5 October 2011. -

Aim To examine the effect of acute sleep deprivation under

light conditions on the expression of two key clock genes,

hPer2 and hBmal1, in peripheral blood mononuclear cells

PBMC and on plasma melatonin and cortisol levels.

Methods Blood samples were drawn from 6 healthy individuals

at 4-hour intervals for three consecutive nights,

including a night of total sleep deprivation second night.

The study was conducted in April-June 2006 at the University

Medical Centre Ljubljana.

Results We found a significant diurnal variation in hPer2

and hBmal1 expression levels under baseline P < 0.001,

F = 19.7, df = 30 for hPer2 and P < 0.001, F = 17.6, df = 30 for

hBmal1 and sleep-deprived conditions P < 0.001, F = 9.2,

df = 30 for hPer2 and P < 0.001, F = 13.2, df = 30 for hBmal1.

Statistical analysis with the single cosinor method revealed

circadian variation of hPer2 under baseline and of hBmal1

under baseline and sleep-deprived conditions. The peak

expression of hPer2 was at 13:55 ± 1:15 hours under baseline

conditions and of hBmal1 at 16:08 ± 1:18 hours under

baseline and at 17:13 ± 1:35 hours under sleep-deprived

conditions. Individual cosinor analysis of hPer2 revealed a

loss of circadian rhythm in 3 participants and a phase shift

in 2 participants under sleep-deprived conditions. The

plasma melatonin and cortisol rhythms confirmed a conventional

alignment of the central circadian pacemaker to

the habitual sleep-wake schedule.

Conclusion Our results suggest that 40-hour acute sleep

deprivation under light conditions may affect the expression

of hPer2 in PBMCs

Sleep deprivation; light exposure; clock genes; hPer2, hBmal1; peripheral blood mononuclear cells



Autor: Pavel Kavčič - ; Institute of Clinical Neurophysiology, Division of Neurology, University Medical Centre, Ljubljana, Slovenia B

Fuente: http://hrcak.srce.hr/



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