Lef1 may contribute to agenesis of the third molars in miceReport as inadecuate

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Tooth agenesis is the most common developmentalanomaly of the human dentition. Epilepsy-like disorder EL mice, which have a 100%incidence of agenesis of the third molars, may be a good model for the geneticstudy of human tooth agenesis. Our previous congenic breeding strategy using ELmice confined a major locus for agenesis of M3, designated am3, within anapproximately 1 Mega base pair Mbp interval on chromosome 3, which containsfive known genes; Lef1, Hadh, Cyp2u1, Sgms2 and Papss1. The aim of this study was to identify the strongest candidatefor am3 among the five genes usingreal-time PCR analysis. The tooth germs of M3 in the bud stage of EL andcontrol mice were dissected out, and total RNA was extracted. In real-time PCRanalysis, a significantly low level of expression of Lef1, which is one of the essential transcription factors for earlytooth development, was observed in M3 of EL mice. In addition, a significantlylow level of expression of Fgf4,which is a direct transcriptional target for LEF1 in early tooth development,was observed in M3 of EL mice. Our results suggest that the cause of M3 agenesisof EL mice may be a low level of Lef1expression in M3 in the bud stage of EL mice.


Hypodontia; Gene Expression; EL Mice

Cite this paper

Shimizu, T. , Ichinosawa, T. , Kiguchi, Y. , Ishida, F. and Maeda, T. 2013 Lef1 may contribute to agenesis of the third molars in mice. Open Journal of Stomatology, 3, 281-286. doi: 10.4236-ojst.2013.35047.

Author: Takehiko Shimizu, Takahiro Ichinosawa, Yuri Kiguchi, Fusae Ishida, Takahide Maeda

Source: http://www.scirp.org/


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