Functional Characterization of Calcineurin Homologs PsCNA1-PsCNB1 in Puccinia striiformis f. sp. tritici Using a Host-Induced RNAi SystemReportar como inadecuado




Functional Characterization of Calcineurin Homologs PsCNA1-PsCNB1 in Puccinia striiformis f. sp. tritici Using a Host-Induced RNAi System - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Calcineurin plays a key role in morphogenesis, pathogenesis and drug resistance in most fungi. However, the function of calcineurin genes in Puccinia striiformis f. sp. tritici Pst is unclear. We identified and characterized the calcineurin genes PsCNA1 and PsCNB1 in Pst. Phylogenetic analyses indicate that PsCNA1 and PsCNB1 form a calcium-calmodulin regulated protein phosphatase belonging to the calcineurin heterodimers composed of subunits A and B. Quantitative RT-PCR analyses revealed that both PsCNA1 and PsCNB1 expression reached their maximum in the stage of haustorium formation, which is one day after inoculation. Using barely stripe mosaic virus BSMV as a transient expression vector in wheat, the expression of PsCNA1 and PsCNB1 in Pst was suppressed, leading to slower extension of fungal hyphae and reduced production of urediospores. The immune-suppressive drugs cyclosporin A and FK506 markedly reduced the germination rates of urediospores, and when germination did occur, more than two germtubes were produced. These results suggest that the calcineurin signaling pathway participates in stripe rust morphogenetic differentiation, especially the formation of haustoria during the early stage of infection and during the production of urediospores. Therefore PsCNA1 and PsCNB1 can be considered important pathogenicity genes involved in the wheat-Pst interaction.



Autor: Hong Zhang, Jun Guo , Ralf T. Voegele, Jinshan Zhang, Yinghui Duan, Huaiyong Luo, Zhensheng Kang

Fuente: http://plos.srce.hr/



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