Aggregatibacter actinomycetemcomitans GroEL Protein Promotes Conversion of Human CD4 T Cells into IFNγ IL10 Producing Tbet Th1 CellsReportar como inadecuado




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One of the heat shock family protein Hsp expressing bacteria is the gram negative, periodontal pathogen Aggregatibacter actinomycetemcomitans Aa. A. actinomycetemcomitans’ Hsp is a 64-kDa GroEL-protein, which has been shown to influence the host cells. In this study we used recombinant A. actinomycetemcomitans GroEL rAaGroEL protein as a model antigen to study GroEL-mediated T cell immune response. Human peripheral mononuclear cells PBMCs, when stimulated with recombinant rAaGroEL, expressed early activation marker CD69 and IL-2R CD25. CD25 and CD69 expressions were higher in CD4+ T cells compared to CD8+ T cells. rAaGroEL-responding CD4+ T cells expressed IL-10, IFNγ and TNFα cytokines. Interestingly, there were also IL-10 and IFNγ double cytokine producing CD4+ T cells. Additionally, IFNγ expressing CD4+ T cells were also T-bet positive. Altogether the results suggest that rAaGroEL protein affects CD4+ T cells to differentiate into IFNγ IL10-secreting T-bet+ Th1 cells.



Autor: Tahsin Saygılı, Semih Can Akıncılar, Bünyamin Akgül, Ayten Nalbant

Fuente: http://plos.srce.hr/



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