Antibacterial activity of plant methanolic extracts on a field isolate of Pseudomonas syringae pvtomato from the Casablanca region MoroccoReportar como inadecuado




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A bacterial field isolate recovered from infected tomato plants in a green-house at Sidi Rehal, a region near Casablanca city Morocco, was identified as the gammaproteobacterium Pseudomonas syringae pv. tomato DC3000 strain, the causal agent of bacterial speck. The bacterial isolate was characterized by morphological, biochemical and molecular biological tests, its growth curves carried out in various culture media, and its phytopathogenicity verified by infection tests. A screening was performed to evaluate the antibacterial activity of methanolic extracts of 12 selected Moroccan plants against the P. syringae pv. tomato DC3000 isolate, and Agar-well diffusion and Broth microdilution methods were used to determine minimum inhibitory and minimum bactericidal concentrations. Among the methanolic extracts tested, only those of Nigella sativa, Geranuim robertianum, Aizoon canariense and Rubia peregrine showed clear inhibitory and bactericidal activities, although the highest values were achieved with N. sativa, a plant used in Morocco as a spice, condiment and medicinal treatment.

KEYWORDS

Bacterial Speck; Bacterial Field Isolate; LOPAT; Pathogenicity Tests; Antibacterial Activity; Methanolic Plant Extracts; Nigella sativa

Cite this paper

Elkhalfi, B. , Essari, A. , Serrano, A. and Soukri, A. 2013 Antibacterial activity of plant methanolic extracts on a field isolate of Pseudomonas syringae pvtomato from the Casablanca region Morocco. Advances in Bioscience and Biotechnology, 4, 1-9. doi: 10.4236-abb.2013.47A2001.





Autor: Bouchra Elkhalfi, Abderrazak Essari, Aurelio Serrano, Abdelaziz Soukri

Fuente: http://www.scirp.org/



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