Isolation of Mannooligosaccharides Corresponding to Antigenic Determinants of Pathogenic Yeast Candida catenulata Cell Wall MannanReportar como inadecuado




Isolation of Mannooligosaccharides Corresponding to Antigenic Determinants of Pathogenic Yeast Candida catenulata Cell Wall Mannan - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

To investigate the chemical structure of cell wall mannan of pathogenic yeast, Candida catenulata IFO 0745 strain, which possess the epitopes of antigenic factors 1, 9, and 34 to genus Candida, we previously performed the two-dimensional nuclear magnetic resonance NMR analysis of this mannan, Fr. C, without the need for harsh procedures. In this study, three oligosaccharides, biose, triose, and tetraose, and mannose were isolated from Fr. C by acetolysis. The results of NMR analysis indicate that the chemical structures of these oligosaccharides were identified to Manα1-2Man, Manα1-2Manα1-2Man, and Manα1-3Manα1-2Manα1-2Man. The most of resultant mannose seems to be originated from the α-1,6-linked mannan backbone which is recognized by antiserum to factor 9. The inhibition assay of slide agglutination reaction between Fr. C and antigenic antibodies using three oligosaccharides indicate that the Manα1-2Manα1-2Man and Manα1-3Manα1-2Manα1-2Man possess domains corresponding to immunodominants of antigenic factors 1 and 34, respectively.

KEYWORDS

Cell Wall Mannan; Antigenic Factor; Candida catenulata; Acetolysis; Oligomannosidic Epitope

Cite this paper

H. Kobayashi, S. Kawakami, Y. Ogawa, N. Shibata and S. Suzuki -Isolation of Mannooligosaccharides Corresponding to Antigenic Determinants of Pathogenic Yeast Candida catenulata Cell Wall Mannan,- Advances in Microbiology, Vol. 3 No. 2, 2013, pp. 222-226. doi: 10.4236-aim.2013.32033.





Autor: Hidemitsu Kobayashi, Susumu Kawakami, Yukiko Ogawa, Nobuyuki Shibata, Shigeo Suzuki

Fuente: http://www.scirp.org/



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