Comparative Analysis of Pathogenicity Assays and PCR for Listeria monocytogenes Isolated from Animal, Raw Food and Environmental Sources in KoreaReportar como inadecuado




Comparative Analysis of Pathogenicity Assays and PCR for Listeria monocytogenes Isolated from Animal, Raw Food and Environmental Sources in Korea - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Results of PCR with oligonucleotide primers were designed from the assembled panel of four potential virulence genes two of internalin gene and two of transcriptional regulator gene. Most of the isolates including reference strains were reactive by PCR, whereas the other strains No.80, 81, and 83 isolated from pork, were non-reactive by PCR. In particular, all pork isolates were PCR-negative for two primers lmo2672 and 2821 sets tested. However, No.82 was positive for lmo1134 primer, and No.84 was positive for lmo2470 of pork isolates. It was observed that all Listeria monocytogenes L. monocytogenes penetrate Vero cells, although the invasion efficiency of each strain varied between 0.5 and 18.9%. When compared in cell assay with PFGE, the results were shown that the mean invasion efficiency for lineage II isolate 2.6% was significantly lower ANOVA-test, p < 0.05 than that for lineage I 12.9% and III isolates 10.3%.

KEYWORDS

PFGE; Vero Cell; Listeria monocytogenes; in Vitro Assay

Cite this paper

S. Park, J. Jung, Y. Oh, J. Lee, S. Ryu, H. Jung, S. Park, M. Song, G. Park, S. Choi, S. Lee, J. Kim, Y. Chae, B. Jung, M. Lee and H. Kim -Comparative Analysis of Pathogenicity Assays and PCR for Listeria monocytogenes Isolated from Animal, Raw Food and Environmental Sources in Korea,- Advances in Microbiology, Vol. 3 No. 2, 2013, pp. 191-199. doi: 10.4236-aim.2013.32029.





Autor: Sanghun Park, Jihun Jung, Younghee Oh, Jibho Lee, Seunghee Ryu, Hyowon Jung, Sunhee Park, Miok Song, Gunyong Park, Sungmin Choi,

Fuente: http://www.scirp.org/



DESCARGAR PDF




Documentos relacionados