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In this paper we present the results of our researchof E. coli cells and cellular components,DNA and protein thioredoxin, using highly resolved sub-Terahertz THz vibrationalspectroscopy. In this combined research, the results from experimental spectroscopyare analyzed via molecular dynamics MD simulation of vibrational modes and absorption spectra from E. coli cells and constituents in the sub-THzrange. Simplified models of DNA macromolecules with a short sequencing have beenconstructed for several E. coli strainswith the goal to predict their absorption spectra. The similarity between spectralcharacteristics of E. coli cells andcellular components observed in experiments helps us to better understand the mechanism of material interaction with THz radiation and to add genetic informationto the characteristic signatures from biological objects. Modeling results supportedby experimental characterization using a spectroscopic sensor prototype developedand built by Vibratess confirm that an optical, label and reagent free techniquecan be used to examine, detect, and identify bacterial cells with high accuracyand selectivity to the level of strains.

KEYWORDS

Bacterial; Strains; MD; Modeling; Experiment; Components; Absorption; Signatures

Cite this paper

Globus, T. , Sizov, I. and Gelmont, B. 2013 Teraherz vibrational spectroscopy of E. coli and molecular constituents: Computational modeling and experiment. Advances in Bioscience and Biotechnology, 4, 493-503. doi: 10.4236-abb.2013.43A065.





Autor: Tatiana Globus, Igor Sizov, Boris Gelmont

Fuente: http://www.scirp.org/



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