Fungal Diversity Is Not Determined by Mineral and Chemical Differences in Serpentine SubstratesReportar como inadecuado




Fungal Diversity Is Not Determined by Mineral and Chemical Differences in Serpentine Substrates - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

The physico-chemical properties of serpentine soils lead to strong selection of plant species. Whereas many studies have described the serpentine flora, little information is available on the fungal communities dwelling in these sites. Asbestos minerals, often associated with serpentine rocks, can be weathered by serpentine-isolated fungi, suggesting an adaptation to this substrate. In this study, we have investigated whether serpentine substrates characterized by the presence of rocks with distinct mineral composition could select for different fungal communities. Both fungal isolation and 454 pyrosequencing of amplicons obtained from serpentine samples following direct DNA extraction revealed some fungal taxa shared by the four ophiolitic substrates, but also highlighted several substrate-specific taxa. Bootstrap analysis of 454 OTU abundances indicated weak clustering of fungal assemblages from the different substrates, which did not match substrate classification based on exchangeable macronutrients and metals. Intra-substrate variability, as assessed by DGGE profiles, was similar across the four serpentine substrates, and comparable to inter-substrate variability. These findings indicate the absence of a correlation between the substrate mineral composition and available cations and the diversity of the fungal community. Comparison of culture-based and culture-independent methods supports the higher taxonomic precision of the former, as complementation of the better performance of the latter.



Autor: Stefania Daghino , Claude Murat, Elisa Sizzano, Mariangela Girlanda, Silvia Perotto

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados