Increased Responsiveness of Peripheral Blood Mononuclear Cells to In Vitro TLR 2, 4 and 7 Ligand Stimulation in Chronic Pain PatientsReportar como inadecuado




Increased Responsiveness of Peripheral Blood Mononuclear Cells to In Vitro TLR 2, 4 and 7 Ligand Stimulation in Chronic Pain Patients - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Glial activation via Toll-like receptor TLR signaling has been shown in animals to play an important role in the initiation and establishment of chronic pain. However, our ability to assess this central immune reactivity in clinical pain populations is currently lacking. Peripheral blood mononuclear cells PBMCs are an accessible source of TLR expressing cells that may mirror similarities in TLR responsiveness of the central nervous system. The aim of this study was to characterize the IL-1β response to various TLR agonists in isolated PBMCs from chronic pain sufferers on and not on opioids and pain-free controls. Venous blood was collected from 11 chronic pain sufferers on opioids ≥ 20 mg of morphine - day, 8 chronic pain sufferers not on opioids and 11 pain-free controls. PBMCs were isolated and stimulated in vitro with a TLR2 Pam3CSK4, TLR4 LPS or TLR7 imiquimod agonist. IL-1β released into the supernatant was measured with ELISA. Significantly increased IL-1β expression was found in PBMCs from chronic pain sufferers on and not on opioids compared with pain-free controls for TLR2 F 6, 277 = 15, P<0.0001, TLR4 F 8, 263 = 3, P = 0.002 and TLR7 F 2,201 = 5, P = 0.005 agonists. These data demonstrate that PBMCs from chronic pain sufferers were more responsive to TLR agonists compared with controls, suggesting peripheral cells may have the potential to become a source of biomarkers for chronic pain.



Autor: Yuen H. Kwok , Mark R. Hutchinson, Melanie G. Gentgall, Paul E. Rolan

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados