Mapping the Mechanome of Live Stem Cells Using a Novel Method to Measure Local Strain Fields In Situ at the Fluid-Cell InterfaceReportar como inadecuado




Mapping the Mechanome of Live Stem Cells Using a Novel Method to Measure Local Strain Fields In Situ at the Fluid-Cell Interface - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

During mesenchymal condensation, the initial step of skeletogenesis, transduction of minute mechanical forces to the nucleus is associated with up or down-regulation of genes, ultimately resulting in formation of the skeletal template and appropriate cell lineage commitment. The summation of these biophysical cues affects the cell-s shape and fate. Here, we predict and measure surface strain, in live stem cells, in response to controlled delivery of stresses, providing a platform to direct short-term structure - function relationships and long-term fate decisions. We measure local strains on stem cell surfaces using fluorescent microbeads coated with Concanavalin A. During delivery of controlled mechanical stresses, 4-Dimensional x,y,z,t displacements of the bound beads are measured as surface strains using confocal microscopy and image reconstruction. Similarly, micro-particle image velocimetry μ-piv is used to track flow fields with fluorescent microspheres. The measured flow velocity gradient is used to calculate stress imparted by fluid drag at the surface of the cell. We compare strain measured on cell surfaces with those predicted computationally using parametric estimates of the cell-s elastic and shear modulus. Finally, cross-correlating stress - strain data to measures of gene transcription marking lineage commitment enables us to create stress - strain - fate maps, for live stem cells in situ. The studies show significant correlations between live stem cell stress - strain relationships and lineage commitment. The method presented here provides a novel means to probe the live stem cell-s mechanome, enabling mechanistic studies of the role of mechanics in lineage commitment as it unfolds.



Autor: Min Jae Song, Susann M. Brady-Kalnay, Sara H. McBride, Polly Phillips-Mason, David Dean, Melissa L. Knothe Tate

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados