The Effect of Resveratrol on Bone Status in Rats with Bile Duct ObstructionReportar como inadecuado

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Objective: We studied the influence of bile duct obstruction BDO after 28 days on bone metabolism status and their modification by resveratrol in male Wistar rats. Methods: The rats were divided into 3 groups: Sham group with laparotomy and vehiculum application, group BDO—bile duct obstruction and vehiculum application, and group RBDO—bile duct obstruction and resveratrol application 10 mg-kg dose of resveratrol orally once daily. The bone mineral density BMD; g-cm2 and body composition were measured by dual energy X-ray absorptiometry. The physiccal strenght of femur was examined by controlled break biomechanical testing. The osteocalcin, procollagen type I N-terminal propeptide PINP and carboxy-terminal collagen crosslinks CTX were analysed by EIA in the bone tissue homogenate. Results: The total BMD was lower in group BDO 0.176 ± 0.005; p = 0.01 and R-BDO 0.181 ± 0.004; p = 0.052 vs Sham 0.209 ± 0.003. BMD of femur diaphysis in BDO group decreased significantly p = 0.01 but not in R-BDO animals p = 0.052. Body fat g, median was lower in BDO 19.0 and R-BDO 26.0 than in Sham 35.0. The force needed for fracture of femurs N significantly decreased in BDO 154 ± 6 and R-BDO 151 ± 13 vs Sham group 208 ± 7. The force needed for neck fracture decreased in BDO 105 ± 3 and R-BDO 115 ± 8 vs Sham 135 ± 9. The osteocalcin decrease in group R-BDO 2.9 ± 0.1 vs Sham 3.7 ± 0.4. Higher PINP-CTX were in BDO 20 ± 5-0.49 ± 0.08 and R-BDO 30 ± 4-0.55 ± 0.07 vs Sham 16 ± 4-0.48 ± 0.11. Conclusions: Our findings suggest that subchronic bile duct obstruction decreased body fat quantity and BMD with decrease of bone rigidity. Resveratrol showed protective effect on liver injury and consequently positive influence on bone tissue.


Bone Metabolism; Rat; Resveratrol; Bile Duct Obstruction

Cite this paper

H. Zivna, S. Micuda, E. Brcakova-Dolezelova and P. Zivny -The Effect of Resveratrol on Bone Status in Rats with Bile Duct Obstruction,- Open Journal of Endocrine and Metabolic Diseases, Vol. 3 No. 1, 2013, pp. 46-51. doi: 10.4236-ojemd.2013.31007.

Autor: Helena Zivna, Stanislav Micuda, Eva Brcakova-Dolezelova, Pavel Zivny



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