Regulation of MUC5AC mucin production by the cell attachment dependent pathway involving integrin β1 in NCI-H292 human lung epithelial cellsReportar como inadecuado




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Mucus hypersecretion in airways is a common pathologicalchange observed in chronic inflammatory diseases and asthma. We investigatedthe new role of cell attachment to the extracellular matrix ECM on theproduction of the airway mucus protein, MUC5AC mucin, in human airwayepithelial cells, NCI-H292. MUC5AC levels of cells cultured on low adhesionplates were 10-fold higher than those of cells cultured on adhesion plates.Cells cultured on bovine serum albumin BSA coated plates, which produce low adhesion conditions, also induced the up-regulation of MUC5AC. Mucin staining byPAS and MUC5AC immunodetection confirmed that mucin proteins were overproducedunder low adhesion conditions. The major adhesion molecule between cells andthe ECM was integrins. A time-course experiment showed that the expressionpatterns of integrin β1 and MUC5ACprotein were inversely proportional. The inhibition of integrin β1 induced an increase in MUC5AC production in cells cultured under adhesion conditions, but not under low adhesionconditions. These results suggested that cell attachment regulates MUC5AC production, which is up-regulated by low adhesion to the ECM, and MUC5ACproduction is inversely proportional to the function of integrin β1.

KEYWORDS

Asthma; Mucin; MUC5AC; Mucus; Integrin

Cite this paper

Iwashita, J. , Hongo, K. , Ito, Y. , Abe, T. and Murata, J. 2013 Regulation of MUC5AC mucin production by the cell attachment dependent pathway involving integrin β1 in NCI-H292 human lung epithelial cells. Advances in Biological Chemistry, 3, 1-10. doi: 10.4236-abc.2013.31001.





Autor: Jun Iwashita, Kaori Hongo, Yuho Ito, Tatsuya Abe, Jun Murata

Fuente: http://www.scirp.org/



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