The Structural Stability of the Endothelial Glycocalyx after Enzymatic Removal of GlycosaminoglycansReportar como inadecuado




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Rationale

It is widely believed that glycosaminoglycans GAGs and bound plasma proteins form an interconnected gel-like structure on the surface of endothelial cells the endothelial glycocalyx layer–EGL that is stabilized by the interaction of its components. However, the structural organization of GAGs and proteins and the contribution of individual components to the stability of the EGL are largely unknown.

Objective

To evaluate the hypothesis that the interconnected gel-like glycocalyx would collapse when individual GAG components were almost completely removed by a specific enzyme.

Methods and Results

Using confocal microscopy, we observed that the coverage and thickness of heparan sulfate HS, chondroitin sulfate CS, hyaluronic acid HA, and adsorbed albumin were similar, and that the thicknesses of individual GAGs were spatially nonuniform. The individual GAGs were degraded by specific enzymes in a dose-dependent manner, and decreased much more in coverage than in thickness. Removal of HS or HA did not result in cleavage or collapse of any of the remaining components. Simultaneous removal of CS and HA by chondroitinase did not affect HS, but did reduce adsorbed albumin, although the effect was not large.

Conclusion

All GAGs and adsorbed proteins are well inter-mixed within the structure of the EGL, but the GAG components do not interact with one another. The GAG components do provide binding sites for albumin. Our results provide a new view of the organization of the endothelial glycocalyx layer and provide the first demonstration of the interaction between individual GAG components.



Autor: Ye Zeng, Eno E. Ebong, Bingmei M. Fu, John M. Tarbell

Fuente: http://plos.srce.hr/



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