Imaging Lung Function in Mice Using SPECT-CT and Per-Voxel AnalysisReportar como inadecuado

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Chronic lung disease is a major worldwide health concern but better tools are required to understand the underlying pathologies. Ventilation-perfusion V-Q single photon emission computed tomography SPECT with per-voxel analysis allows for non-invasive measurement of regional lung function. A clinically adapted V-Q methodology was used in healthy mice to investigate V-Q relationships. Twelve week-old mice were imaged to describe normal lung function while 36 week-old mice were imaged to determine how age affects V-Q. Mice were ventilated with Technegas™ and injected with 99mTc-macroaggregated albumin to trace ventilation and perfusion, respectively. For both processes, SPECT and CT images were acquired, co-registered, and quantitatively analyzed. On a per-voxel basis, ventilation and perfusion were moderately correlated R = 0.58±0.03 in 12 week old animals and a mean logV-Q ratio of −0.07±0.01 and standard deviation of 0.36±0.02 were found, defining the extent of V-Q matching. In contrast, 36 week old animals had significantly increased levels of V-Q mismatching throughout the periphery of the lung. Measures of V-Q were consistent across healthy animals and differences were observed with age demonstrating the capability of this technique in quantifying lung function. Per-voxel analysis and the ability to non-invasively assess lung function will aid in the investigation of chronic lung disease models and drug efficacy studies.

Autor: Brian N. Jobse, Rod G. Rhem, Cory A. J. R. McCurry, Iris Q. Wang, N. Renée Labiris



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