Temporal Changes in Cell Marker Expression and Cellular Infiltration in a Controlled Cortical Impact Model in Adult Male C57BL-6 MiceReportar como inadecuado




Temporal Changes in Cell Marker Expression and Cellular Infiltration in a Controlled Cortical Impact Model in Adult Male C57BL-6 Mice - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Background

Traumatic injury to the central nervous system CNS triggers a robust inflammatory response that leads to axonal damage and secondary degeneration of spared tissue. In contrast, some immune responses have neuroprotective effects. However, detailed information regarding the dynamics of immune responses after traumatic CNS injury is still unavailable.

Methods

In the present study, changes in the immune cells present in the injured brain, spleen, and cervical lymph nodes CLNs, which are draining lymphatic organs from the CNS, were analyzed after controlled cortical impact CCI by flow cytometry and immunohistochemistry.

Results

The number of neutrophils and macrophages that infiltrated the injured brain immediately increased 1 d post-injury and declined rapidly thereafter. In the injured brain, resident microglia showed a bimodal increase during the first week and in the chronic phase ≥3 weeks after injury. Increase in the Iba-1+ microglia-macrophages was observed around the injured site. Morphologic analysis showed that Iba-1+ cells were round at 1 week, whereas those at 3 weeks were more ramified. Furthermore, CD86+-CD11b+ M1-like microglia increased at 4 weeks after CCI, whereas CD206+-CD11b+ M2-like microglia increased at 1 week. These results suggest that different subsets of microglia increased in the acute and chronic phases after CCI. Dendritic cells and T cells increased transiently within 1 week in the injured brain. In the CLNs and the spleen, T cells showed dynamic changes after CCI. In particular, the alteration in the number of T cells in the CLNs showed a similar pattern, with a 1-week delay, to that of microglia in the injured brain.

Conclusion

The data from this study provide useful information on the dynamics of immune cells in CNS injuries.



Autor: Xuemei Jin, Hiroshi Ishii, Zhongbin Bai, Takahide Itokazu, Toshihide Yamashita

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados