Characterization of Immune Responses Induced by Immunization with the HA DNA Vaccines of Two Antigenically Distinctive H5N1 HPAIV IsolatesReportar como inadecuado




Characterization of Immune Responses Induced by Immunization with the HA DNA Vaccines of Two Antigenically Distinctive H5N1 HPAIV Isolates - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

The evolution of the H5N1 highly pathogenic avian influenza virus HPAIV has resulted in high sequence variations and diverse antigenic properties in circulating viral isolates. We investigated immune responses induced by HA DNA vaccines of two contemporary H5N1 HPAIV isolates, A-bar-headed goose-Qinghai-3-2005 QH and A-chicken-Shanxi-2-2006 SX respectively, against the homologous as well as the heterologous virus isolate for comparison. Characterization of antibody responses induced by immunization with QH-HA and SX-HA DNA vaccines showed that the two isolates are antigenically distinctive. Interestingly, after immunization with the QH-HA DNA vaccine, subsequent boosting with the SX-HA DNA vaccine significantly augmented antibody responses against the QH isolate but only induced low levels of antibody responses against the SX isolate. Conversely, after immunization with the SX-HA DNA vaccine, subsequent boosting with the QH-HA DNA vaccine significantly augmented antibody responses against the SX isolate but only induced low levels of antibody responses against the QH isolate. In contrast to the antibody responses, cross-reactive T cell responses are readily detected between these two isolates at similar levels. These results indicate the existence of original antigenic sin OAS between concurrently circulating H5N1 HPAIV strains, which may need to be taken into consideration in vaccine development against the potential H5N1 HPAIV pandemic.



Autor: Yulong Gao , Zhiyuan Wen , Ke Dong, Gongxun Zhong, Xiaomei Wang, Zhigao Bu, Hualan Chen, Ling Ye, Chinglai Yang

Fuente: http://plos.srce.hr/



DESCARGAR PDF




Documentos relacionados