The Pro-Inflammatory Cytokine IL-22 Up-Regulates Keratin 17 Expression in Keratinocytes via STAT3 and ERK1-2Reportar como inadecuado




The Pro-Inflammatory Cytokine IL-22 Up-Regulates Keratin 17 Expression in Keratinocytes via STAT3 and ERK1-2 - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Background

To investigate the regulation of K17 expression by the pro-inflammatory cytokine IL-22 in keratinocytes and its important role in our previously hypothesized -K17-T cell-cytokine autoimmune loop- in psoriasis.

Materials and Methods

K17 expression was examined in the IL-22-treated keratinocytes by real-time quantitative PCR, ELISA, Western blot and Immunofluorescence. In addition, the signaling pathways involved in K17 regulation were investigated with related inhibitors and siRNAs. In addition, K17 expression was examined in the epidermis of IL-22-injected mouse skin.

Results

IL-22-induced K17 expression was confirmed in keratinocytes and the epidermis of IL-22-injected mouse skin at both mRNA and protein levels, which is an important complement to the autoimmune loop. We further investigated the regulatory mechanisms and found that both STAT3 and ERK1-2 were involved in the up-regulation of K17 expression induced by IL-22.

Conclusion

IL-22 up-regulates K17 expression in keratinocytes in a dose-dependent manner through STAT3- and ERK1-2-dependent mechanisms. These findings indicated that IL-22 was also involved in the K17-T cell-cytokine autoimmune loop and may play an important role in the progression of psoriasis.



Autor: Wei Zhang , Erle Dang , Xiaowei Shi, Liang Jin, Zhenzhen Feng, Lei Hu, Yan Wu, Gang Wang

Fuente: http://plos.srce.hr/



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