Augmented TLR2 Expression on Monocytes in both Human Kawasaki Disease and a Mouse Model of Coronary ArteritisReport as inadecuate

Augmented TLR2 Expression on Monocytes in both Human Kawasaki Disease and a Mouse Model of Coronary Arteritis - Download this document for free, or read online. Document in PDF available to download.


Kawasaki disease KD of unknown immunopathogenesis is an acute febrile systemic vasculitis and the leading cause of acquired heart diseases in childhood. To search for a better strategy for the prevention and treatment of KD, this study compared and validated human KD immunopathogenesis in a mouse model of Lactobacillus casei cell wall extract LCWE-induced coronary arteritis.


Recruited subjects fulfilled the criteria of KD and were admitted for intravenous gamma globulin IVIG treatment at the Kaohsiung Chang Gung Memorial Hospital from 2001 to 2009. Blood samples from KD patients were collected before and after IVIG treatment, and cardiovascular abnormalities were examined by transthoracic echocardiography. Wild-type male BALB-c mice 4-week-old were intraperitoneally injected with LCWE 1 mg-mL to induce coronary arteritis. The induced immune response in mice was examined on days 1, 3, 7, and 14 post injections, and histopathology studies were performed on days 7 and 14.


Both human KD patients and LCWE-treated mice developed coronary arteritis, myocarditis, valvulitis, and pericarditis, as well as elevated plasma levels of interleukin IL-2, IL-6, IL-10, monocyte chemoattractant protein MCP-1, and tumor necrosis factor TNF-α in acute phase. Most of these proinflammatory cytokines declined to normal levels in mice, whereas normal levels were achieved in patients only after IVIG treatment, with a few exceptions. Toll-like receptor TLR-2, but not TLR4 surface enhancement on circulating CD14+ monocytes, was augmented in KD patients before IVIG treatment and in LCWE-treated mice, which declined in patients after IVIG treatment.


This result suggests that that not only TLR2 augmentation on CD14+ monocytes might be an inflammatory marker for both human KD patients and LCWE-induced CAL mouse model but also this model is feasible for studying therapeutic strategies of coronary arteritis in human KD by modulating TLR2-mediated immune activation on CD14+ monocytes.

Author: I-Chun Lin, Ho-Chang Kuo, Ying-Jui Lin, Feng-Shen Wang , Lin Wang, Shun-Chen Huang, Shao-Ju Chien, Chien-Fu Huang, Chih-Lu Wang,



Related documents