Dufulin Activates HrBP1 to Produce Antiviral Responses in TobaccoReportar como inadecuado




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Background

Dufulin is a new antiviral agent that is highly effective against plant viruses and acts by activating systemic acquired resistance SAR in plants. In recent years, it has been used widely to prevent and control tobacco and rice viral diseases in China. However, its targets and mechanism of action are still poorly understood.

Methodology-Principal Findings

Here, differential in-gel electrophoresis DIGE and classical two-dimensional electrophoresis 2-DE techniques were combined with mass spectrometry MS to identify the target of Dufulin. More than 40 proteins were found to be differentially expressed ≥1.5 fold or ≤1.5 fold upon Dufulin treatment in Nicotiana tabacum K326. Based on annotations in the Gene Ontology GO and the Kyoto Encyclopedia of Genes and Genomes KEGG databases, these proteins were found to be related to disease resistance. Directed acyclic graph DAG analysis of the various pathways demonstrated harpin binding protein-1 HrBP1 as the target of action of Dufulin. Additionally, western blotting, semi-quantitative reverse transcription polymerase chain reaction RT-PCR, and real time PCR analyses were also conducted to identify the specific mechanism of action of Dufulin. Our results show that activation of HrBP1 triggers the salicylic acid SA signaling pathway and thereby produces antiviral responses in the plant host. A protective assay based on lesion counting further confirmed the antiviral activity of Dufulin.

Conclusion

This study identified HrBP1 as a target protein of Dufulin and that Dufulin can activate the SA signaling pathway to induce host plants to generate antiviral responses.



Autor: Zhuo Chen, Mengjiao Zeng, Baoan Song , Chengrui Hou, Deyu Hu, Xiangyang Li, Zhenchao Wang, Huitao Fan, Liang Bi, Jiaju Liu, Danda

Fuente: http://plos.srce.hr/



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