Internal Ribosomal Entry Site-Mediated Translation Is Important for Rhythmic PERIOD1 ExpressionReportar como inadecuado




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The mouse PERIOD1 mPER1 plays an important role in the maintenance of circadian rhythm. Translation of mPer1 is directed by both a cap-dependent process and cap-independent translation mediated by an internal ribosomal entry site IRES in the 5′ untranslated region UTR. Here, we compared mPer1 IRES activity with other cellular IRESs. We also found critical region in mPer1 5′UTR for heterogeneous nuclear ribonucleoprotein Q HNRNPQ binding. Deletion of HNRNPQ binding region markedly decreased IRES activity and disrupted rhythmicity. A mathematical model also suggests that rhythmic IRES-dependent translation is a key process in mPER1 oscillation. The IRES-mediated translation of mPer1 will help define the post-transcriptional regulation of the core clock genes.



Autor: Kyung-Ha Lee, Sung-Hoon Kim, Do-Yeon Kim, Seunghwan Kim, Kyong-Tai Kim

Fuente: http://plos.srce.hr/



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